Identification of viruses associated with honeybees (Apis mellifera) - SRA

SRX26438594: Identification of viruses associated with honeybees (Apis mellifera)
1 ILLUMINA (Illumina NovaSeq X Plus) run: 9M spots, 2.7G bases, 777.6Mb downloads

Design: Individual insect was homogenized using a 1.5ml tube and pestles in 800l of SM Buffer (0.1M NaCl, 10mM MgSO4, 50 mM Tris-HCl pH 7.4]). This was then spun down at 8,000 rpm for 5 minutes and 200l of the supernatant were used for DNA extraction using the High Pure Viral Nucleic Acid kit (Roche Diagnostic, USA). The DNA was then used as a template for rolling circle amplification (RCA) using the TempliPhi 2000 kit (ThermoFisher Scientific, USA). RCA products were then combined with the viral DNA at a 1:1 ratio and used to generate Illumina sequencing libraries using the Illumina DNA library prep kit (Illumina, USA). These libraries were sequenced on an Illumina NovaSeq X plus (Illumina, USA) sequencing platform.

Submitted by: Arizona State University

Study: Viral metagenomics of bees (Apis mellifera and Nomia sp.) and hoverflies

PRJNA1171949 • SRP538269 • All experiments • All runs

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Identification of viruses associated with honeybees (Apis mellifera), alkali bees (Nomia sp.) and hoverflies (Syrphoidea)

Sample:

SAMN44294403 • SRS22955108 • All experiments • All runs

Organism: viral metagenome

Library:

Name: SR_HBPW1_4

Instrument: Illumina NovaSeq X Plus

Strategy: WGS